Liquid Culture
Supplies
- Dry malt extract
- Corn syrup
- Peptone, bacteriological
- Wide mouth quart jars with LC lids (see below)
- Stir bar
LC Lids
Plastic lids with silicone seal ring, self healing injection port, and PTFE syringe filter (13mm 22um)
Drill two holes in the lids: ½ inch bit for SHIP, 5/32 inch bit for PTFE filter
Push the SHIP and PTFE filter in and seal from bottom with high temp silicone, wait 24 hours to dry completely before use.
Process
- Add 500 ml water into a jar with LC lid (see below)
- Add 12 ml / 20g corn syrup
- Add 1 gram of dry malt extract
- Add 1 gram of peptone (soy)
- Add stir bar
- Pressure sterilize for 25 minutes at 15 psi (turn off stove at 20 minutes, open valve at 0 psi if using All American)